On Friday 17th 2023, the University of Otago team (from Gemmell’s lab) alongside the Marine Biosecurity Toolbox had a booth at the ‘All the small things´ event hosted by the Otago Museum and led by Genomics Aotearoa. The event goal was to showcase the environmental DNA research happening at the University of Otago to incoming students, their families and the community.
The DETECT team representatives ( Jo Stanton, Jackson Treece and Benjamín Durán-Vinet) set up a portable isothermal amplification technique demonstration known as LAMP (Loop-mediated isothermal amplification) to portray a rapid, portable, simple and highly specific environmental DNA detection tool that could be used by non-scientific users. They also spoke about other potential eDNA detection tools, such a Recombinase Polymerase Amplification (RPA) and Clustered Regularly Interspaced Palindromic Short Repeats (CRISPR).
The booth had several enthusiastic non-scientific volunteers that listened about the importance of eDNA and LAMP potential. They then went through a quick walkthrough of micropipetting to perform a LAMP reaction by themselves by adding 2 uL of sample into the mastermix. Moreover, only 30 minutes later, they were able to directly witness the output of their great work, which worked perfectly. Therein, showcasing its ease-to-use, portability, and accessibility for in-field applications. They were thrilled and happy to see their results and how the Marine Biosecurity Tool is developing new and better tools to keep our marine ecosystem safe.
